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Image Search Results
Journal: Redox Biology
Article Title: Nox4 promotes endothelial differentiation through chromatin remodeling
doi: 10.1016/j.redox.2022.102381
Figure Lengend Snippet: Nox4 promotes angiogenic potential in the course of differentiation . (A&B) VEGF-induced effects in cells, derived from embryonic bodies at day 7 of differentiation in (A) tube formation assay with statistics for number of tubes and tube length and (B) Spheroid outgrowth assay with statistics for sprouting & branching with and without 3 μM H 2 O 2 (n = 3–8; *p<0.05 WT vs. Nox4 −/− ). (C) Integration of purified WT- or Nox4 −/− -miPSC-ECs into newly formed endothelial networks in Matrigel plugs 3 weeks after implantation into SCID-mice. (green: newly formed vessel staine with Isolectin B4; red: cells still present in the plug stained with Vybrant Dil; blue: integrated cells detected by algorithm); fibroblasts used as negative control and HUVECs as positive control (n = 5; *p<0.05 WT vs. Nox4 −/− ). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: WT & Nox4 knockout endothelial cells were isolated at day 14 of differentiation with
Techniques: Derivative Assay, Tube Formation Assay, Purification, Staining, Negative Control, Positive Control